Electrophoresis is a method used to separate fragments of DNA or RNA based on their size (length*) using an electric current. It has applications in processes such as genetic fingerprinting and disease diagnosis. DNA and RNA are negatively charged which means they will move towards a positive electrode. This technique can be used for proteins,... Continue Reading →
So far in the genetic engineering journey we have learnt how to isolate or produce a DNA fragment, and amplify it in vitro using PCR. Bacterial transformation is a method to amplify a DNA fragment in vivo (in a living organism), and to engineer bacteria to produce a protein. In this article we will look... Continue Reading →
Once a DNA fragment has been isolated or produced (see the last article), the next step is often to produce many copies of it. One common way to do this is to use the polymerase chain reaction, which is a fairly simple process that can be automated. In this article we will look in detail... Continue Reading →
This article begins a new series about gene technologies. Many gene technologies, especially recombinant DNA technologies, begin with the need to isolate or make DNA fragments. We will look at three different ways that this can be achieved. Restriction enzymes This method allows DNA fragments to be "cut out" of existing DNA. Restriction enzymes, sometimes... Continue Reading →
