HIV (human immunodeficiency virus) is a virus which can eventually lead to development of AIDS (acquired immunodeficiency syndrome). However, it is not the virus itself which causes AIDS symptoms; HIV weakens the immune system, meaning other infections can take hold more easily. In this article we will look at HIV structure, replication, and development of... Continue Reading →
Enzyme-Controlled Reactions – Practical Skills Ep 1
The aim of this practical is to investigate the rate of enzyme-controlled reactions. The details of the practical itself vary depending on what the enzyme, substrate, and product are. Let's use catalase as an example. Catalase is an intracellular enzyme which catalyses the conversion of toxic hydrogen peroxide (the substrate) into oxygen and water (the... Continue Reading →
Bacterial Transformation – Gene Technologies Ep 3
So far in the genetic engineering journey we have learnt how to isolate or produce a DNA fragment, and amplify it in vitro using PCR. Bacterial transformation is a method to amplify a DNA fragment in vivo (in a living organism), and to engineer bacteria to produce a protein. In this article we will look... Continue Reading →
Polymerase Chain Reaction (PCR) – Gene Technologies Ep 2
Once a DNA fragment has been isolated or produced (see the last article), the next step is often to produce many copies of it. One common way to do this is to use the polymerase chain reaction, which is a fairly simple process that can be automated. In this article we will look in detail... Continue Reading →
Producing DNA Fragments – Gene Technologies Ep 1
This article begins a new series about gene technologies. Many gene technologies, especially recombinant DNA technologies, begin with the need to isolate or make DNA fragments. We will look at three different ways that this can be achieved. Restriction enzymes This method allows DNA fragments to be "cut out" of existing DNA. Restriction enzymes, sometimes... Continue Reading →
Control of Blood Glucose – Homeostasis Ep 3
Control of blood glucose is very important. Sufficient glucose must be delivered to cells for respiration, but too much glucose can eventually result in coma or even death. The concentration of glucose is affected by factors such as eating and exercise, and must be brought back to the normal level of about 4mmol/L to 5.5mmol/L.... Continue Reading →
Feedback Mechanisms – Homeostasis Ep 1
Homeostasis is the maintenance of a stable internal environment. The conditions inside the body must be stable so that cells can function properly. For example, if the temperature or pH are too high, enzymes can become denatured. It is also important to maintain blood glucose concentration and water potential, which we will look at in... Continue Reading →
The Cell Cycle and DNA Replication – Cell Division Ep 1
All the cells in multicellular organisms which are capable of division go through a cell cycle - a sequence of phases which incorporates cell growth, DNA replication, and cell division by mitosis. However, remember that not all cells are capable of division e.g. red blood cells. The Cell Cycle The cell cycle begins just after... Continue Reading →
Limiting Factors – Photosynthesis Ep 3
The environmental conditions must be just right for photosynthesis to take place at the fastest possible rate. Today we're going to take a look at how light intensity, temperature and carbon dioxide (CO2) concentration can affect and limit the rate of photosynthesis. They will also affect plant growth, because photosynthesis produces glucose for respiration. Plants... Continue Reading →
Coenzymes and Cofactors – Enzymes Ep 4
So far in this series we have looked at how enzymes work, what can affect the rate at which they work, and how their action can be inhibited. Today we are looking at coenzymes and cofactors: non-proteins that are needed in order for some enzymes to be able to catalyse their reaction. Coenzymes Coenzymes are... Continue Reading →